The United States won the 2018 World Softball Championships, guaranteeing them a spot in the six-team Tokyo Olympics field. On October 6, 2019, USA Softball became the first U.S. national governing body to announce its entire roster of athletes for the Tokyo Olympics. Last May, USA Softball confirmed that, despite the Olympic postponement to 2021 due to the Covid-19 pandemic, the roster named in 2019 will indeed be the group that will compete in Tokyo.

The 15-player squad is:

Monica Abbott

Ali Aguilar

Valerie Arioto

Ally Carda

Amanda Chidester

Rachel Garcia

Haylie McCleney

Michelle Moultrie

Dejah Mulipola

Aubree Munro

Bubba Nickles

Cat Osterman

Janie Reed

Delaney Spaulding

Kelsey Stewart

The three alternates are:

Taylor Edwards 

Hannah Flippen

Keilani Ricketts

Team USA will compete against the other five qualified nations: Australia, Canada, Italy, Japan and ​​​​​Mexico.